- Product name: DL-Serine Assay Kit
- Detection method: Fluorescent
- Sample type: Saliva, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate, Tissue Homogenate, Cerebral Spinal Fluid
- Sensitivity: < 1 µM
DL-Serine Assay Kit (ab241027) allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 535/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable, and can detect less than 1 μM Serine.
Specificity for detection of L- or D-Serine (SER) over other amino acids. At a 5-fold molar excess (10 nmoles) versus SER isomers (each 2 nmoles), L-threonine (THR) contributes ≤15% interference, L-cysteine (CYS) contributes ≤10% interference and all other amino acids contribute ≤5%.
Estimation of total, D- and L-Serine in pooled normal human serum and CSF (10 µL). L-Serine concentrations for serum and CSF samples were 137.4 ± 9.06 µM and 56.01 ± 2.93 µM, whereas D-Serine concentrations were 2.37 ± 0.36 µM and 3.46 ± 0.21 µM, respectively. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.
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