Which steps are essential for the formation of functional legume nodules?

Nodulation is reviewed when it comes to the phenotypes proposed by Vincent (1980). Particular person legumes could also be infectible by a number of of the three bacterial genera (collectively referred to as rhizobia) Rhizobium, Bradyrhizobium, or Azorhizobium. The kind of an infection course of by which rhizobia acquire entry is basically ruled by the host genotype. Along with the extensively studied root-hair pathway, infections could also be related to lateral root emergence or happen between root epidermal cells. The precise chemical and bodily nature of the foundation hair/epidermal cell wall is prone to be a essential consider figuring out whether or not infections can proceed.

Along with differing with species, wall composition could also be influenced by soil chemical (e.g. Ca2+ ) and biotic components (e.g. micro organism). Rhizobial options important for an infection embody explicit floor polysaccharides and the induction of nodulation genes by plant root exudates. Neither of those is prone to be a significant barrier to the extension of nodulation to new hosts. Dissemination of rhizobia inside creating nodules could also be intercellular, through an infection threads or by division of a small quantity of contaminated cells. All practical symbioses finally have ‘intracellular’ micro organism, within the sense that rhizobia are geographically situated inside the boundary of the host cell partitions.

Nevertheless, they continue to be extracellular within the sense that they’re at all times confined by a membrane which is basically of host cell origin. In some genera they’re additionally surrounded by an infection thread partitions, in all probability modified types of ‘invasive’ an infection thread partitions, which permit differentiation of rhizobia into the nitrogen-fixing type. Thus, pure, practical, symbioses might (a) by no means contain a stage through which micro organism are confined inside tubular an infection threads or (b) by no means launch micro organism from an infection threads. These options are decided by host genotype. The one characteristic of legume nodules thus far discovered by no means to fluctuate is the stem-like character of a peripheral vascular system.

This contrasts with the central vascular system of actinorhizas and the rhizobial-induced nodules on the Ulmaceous genus Parasponia. Though of nice intrinsic curiosity, this character is unlikely to current an insurmountable barrier to the extension of nodulation to new species. Different options, similar to the power to provide haemoglobin at the moment are identified to the within the genetic make-up of many larger vegetation. The invention of the big selection of nodule constructions occurring in nature, along with work on mutant rhizobia which can bypass essential levels within the nodulation course of, counsel varied methods through which the extension of nodulation to non-nodulated legumes and to different (initially no less than, dicotyledonous) vegetation could also be engineered.

Coronavirus consciousness, confinement stress, and psychological well being: Proof from Honduras, Chile, Costa Rica, Mexico and Spain

The commonest stress domains correspond to household (22.97%), monetary (22.53%), educational (16.47%), leisure time constraints (14.23%), well being (12.48%), peer group (7.63%), and non secular considerations (3.69%). These domains are considerably related to the respondent’s nation, intercourse, employment standing, and being or not a well being employee. Respondents who reported confinement stress additionally reported larger scores in anxiousness, despair, and somatization. The World Severity Index was considerably predicted by confinement stress, well being, educational, and leisure time-related stress, intercourse, age, being a well being employee, COVID-19 Private Concern, and Perceived Seriousness.

Non-significant predictors had been employment standing, the variety of folks at residence, presence of older adults and youngsters at residence, monetary, peer group, household, and non secular considerations; the regression mannequin had an R2 of 0.26. Comparable analyses had been carried out for somatization, despair, and anxiousness subscales. The COVID-19 pandemic has opposed results on the psychological well being of the final inhabitants, significantly relating to anxiousness, despair, and somatization. Particular populations, similar to ladies and healthcare employees, are at explicit danger of struggling a deterioration in psychological wellbeing. The implications of the examine for public coverage are mentioned.

Which steps are essential for the formation of functional legume nodules?

Evaluation of human bioengineered cardiac tissue operate in hypoxic and re-oxygenized environments to grasp practical restoration in coronary heart failure

Myocardial restoration is likely one of the targets for coronary heart failure remedy. A non-negligible quantity of coronary heart failure with diminished ejection fraction (EF) sufferers expertise myocardial restoration by way of remedy. Though myocardial hypoxia has been reported to contribute to the development of coronary heart failure even in non-ischemic cardiomyopathy, the connection between contractile restoration and re-oxygenation and its underlying mechanisms stay unclear. The current examine investigated the results of hypoxia/re-oxygenation on bioengineered cardiac cell sheets-tissue operate and the underlying mechanisms.

Hypoxia (four days) elicited progressive decreases in contractile pressure, most contraction velocity, most leisure velocity, Ca2+ transient amplitude and ATP degree, however sarcomere construction and cell quantity weren’t affected. Re-oxygenation (eight days) after hypoxia (four days) was related to progressive will increase in contractile pressure, most contraction velocity and leisure time to the same extent ranges of steady normoxia group, whereas most leisure velocity was nonetheless considerably low even after re-oxygenation. Ca2+ transient magnitude, cell quantity, sarcomere construction and ATP degree after re-oxygenation had been just like these within the steady normoxia group.

Recombinant Zea Mays Casein Kinase 2 alpha

7-04233 20µg Ask for price

Zea Mays Casein Kinase 2 alpha Protein (Recombinant)

20-abx073023
  • EUR 1345.20
  • EUR 2364.00
  • EUR 777.60
  • 10 ug
  • 20 ug
  • 3 µg

Recombinant (E.Coli) Zea Mays Casein Kinase 2 alpha

RP-668 3 ug
EUR 708

CK2a Casein Kinase 2 alpha Zea Mays Recombinant Protein

PROTP28523 Regular: 10ug
EUR 1198.8
Description: Casein Kinase 2 alpha Zea Mays Recombinant is a non-glycosylated polypeptide having a molecular mass of 39.2 kDa. ;Casein Kinase 2 is purified by proprietary chromatographic techniques.

Recombinant other CK2a Zea Mays Protein, Untagged, E.coli-10ug

QP11433-10ug 10ug
EUR 1034.4

Recombinant other CK2a Zea Mays Protein, Untagged, E.coli-20ug

QP11433-20ug 20ug
EUR 1873.2

Recombinant other CK2a Zea Mays Protein, Untagged, E.coli-3ug

QP11433-3ug 3ug
EUR 555.6

TesA, E. coli esterase

0306-1 100 Units
EUR 505.2

E. coli Topoisomerase IV

TG2000EC-1 250 units
EUR 537.6

E. coli DNA Gyrase

TG2000G-1 100 units
EUR 388.8

Streptavidin-Coated Strip Microwell Plate (Flat) 

R-1102
  • EUR 361.02
  • EUR 204.60
  • 5/pk
  • 5/pk

Recombinant Nucleosome (E. coli-derived)

52038-1 100 µg
EUR 505
Description: Recombinant human nucleosome consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-137(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.

Recombinant purified >98% (E. Coli) Protein A

PRTA11-R-1 1 mg
EUR 169.2

Recombinant (E. coli) Pasteurella multocida outer membrane lipoprotein

VacJ15-R-10 10 ug
EUR 416.4

E. coli RecA Protein

01-001 200 ug
EUR 246
Description: The E. coli RecA Protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-007 50 ug
EUR 574.8
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-008 250 ug
EUR 1420.8
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-009 50 ug
EUR 574.8
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-010 250 ug
EUR 1420.8
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-011 50 ug
EUR 574.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-012 250 ug
EUR 1420.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

RecA Protein E. coli

M1231-200 each
EUR 242.4

E. coli Syndecan 1 (SDC1) Protein

20-abx263542
  • EUR 994.80
  • EUR 493.20
  • EUR 644.40
  • 10 ug
  • 2 µg
  • 5 ug

Recombinant (E. coli) Outer membrane protein C Recombinant Protein (ompC/omp1b/porin, 22-367 aa, E. coli, >95%)

OMPC15-R-50 25 ug
EUR 343.2

Escherichia coli (E. coli) Antibody

abx415692-01mg 0.1 mg
EUR 760.8

Escherichia coli (E. coli) Antibody

abx415712-1ml 1 ml
EUR 727.2

Escherichia coli (E. coli) Antibody

abx411320-1ml 1 ml
EUR 610.8

Diphosphoryl Lipid A (E. coli K12 D31m4)

AV-7040-1 1 mg
EUR 634.8

E. Coli Recombinant Thioredoxin 1

6329-50 each
EUR 483.6

E. coli antibody

10-E09C 100 ug
EUR 159.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10A 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10B 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

20C-CR1244GP 1 ml
EUR 561.6
Description: Goat polyclonal E. coli antibody

E. coli antibody

20-ER13 1 ml
EUR 426
Description: Rabbit polyclonal E. coli antibody

E. coli antibody

10C-CR1244M1 100 ug
EUR 489.6
Description: Mouse monoclonal E. coli antibody

E. coli Antibody

abx021669-1mg 1 mg
EUR 1554

E. coli Antibody

abx021670-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx021671-02ml 0.2 ml
EUR 594

E. coli Antibody

abx021672-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx021673-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx022970-1ml 1 ml
EUR 878.4

E. coli Antibody

abx022971-1ml 1 ml
EUR 1161.6

E. coli Antibody

abx022975-1ml 1 ml
EUR 718.8

E. coli Antibody

abx022977-1ml 1 ml
EUR 811.2

E. coli GroL

DAG1704 100 ug
EUR 1098

E. coli GroS

DAG1705 100 ug
EUR 1098

E. coli DsbA

DAG1712 100 ug
EUR 1098

E. coli GrpE

DAG1714 100 ug
EUR 1232.4

Lipopolysaccharides (LPS) from E. coli (strain K-235) purified

LPS12-1 1 mg
EUR 270

Lipopolysaccharides (LPS) from E. coli (strain 0157:H7) purified

LPS15-1 1 mg
EUR 270

E. coli Topo IV Drug Screening Kit (20ug kDNA)

TG1008-1 100 assays
EUR 388.8

KRAS (G12R), Isoform A, His-Tag (E. coli-derived)

100841-1 100 µg
EUR 320
Description: Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-186, with G12R mutation and N-terminal His-tag, expressed in an E. coli expression system. MW=23 kDa.

Recombinant (E. coli) Apical membrane antigen 1 mosaic protein (>95%, His-tag protein, P.falciparum)

AMA15-R-10 10 ug
EUR 416.4

E. coli O157 Verotoxin 1 (VT1) Protein

abx061526-100ug 100 ug
EUR 1212

E. coli Superoxide Dismutase 1 Protein [His]

DAG2437 100 µg
EUR 988.8

Recombinant HSV-1 gD Protein (E. coli)

VAng-0550Lsx-inquire inquire Ask for price
Description: The E. coli derived recombinant protein contains the HSV-1 gD immunodominant regions. MW 40.6 kDa, 1.00 mg/mL.

Escherichia coli (E. coli) Antibody (Biotin)

abx415713-1ml 1 ml
EUR 861.6

Escherichia coli (E. coli) Antibody (Biotin)

abx411321-1ml 1 ml
EUR 710.4

Escherichia coli (E. coli) Antibody (FITC)

abx411322-1ml 1 ml
EUR 610.8

E. Coli O 157 Protein

abx061475-01ml 0.1 ml
EUR 661.2

Escherichia coli Outer membrane protein C (ompC)

1-CSB-EP356994ENV
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Outer membrane protein C(ompC) expressed in E.coli

Escherichia coli Outer membrane protein C (ompC)

1-CSB-EP356994ENVa0
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Outer membrane protein C (ompC) expressed in E.coli

Escherichia coli Outer membrane protein C (ompC)

1-CSB-EP356994ENVe1
  • EUR 872.40
  • EUR 510.00
  • EUR 2331.60
  • EUR 1266.00
  • EUR 1602.00
  • EUR 615.60
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Outer membrane protein C(ompC) expressed in E.coli

Escherichia coli Outer membrane protein F (ompF)

1-CSB-EP365808ENV
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Outer membrane protein F(ompF) expressed in E.coli

ZYCY10P3S2T E. coli Minicircle producer strain (competent, ready to transform)

MN900A-1 5 vials
EUR 733.2

Lipopolysaccharides (LPS) from E. coli (strain O26:B6) gel purified

LPS16-1 1 mg
EUR 270

Lipopolysaccharides (LPS) from E. coli (strain O55:B5) gel purified

LPS17-1 1 mg
EUR 270

E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)

TG1007-1 100 assays
EUR 388.8

Escherichia coli Outer membrane protein A (ompA)

1-CSB-CF364256ENV(A4)
  • EUR 1560.00
  • EUR 688.80
  • EUR 996.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Escherichia coli Outer membrane protein A(ompA) expressed in in vitro E.coli expression system

E. coli STX 1 & 2 Antibody

abx021692-1mg 1 mg
EUR 1612.8

E. coli DnaK (aa 1 - 638)

DAG1707 100 µg
EUR 1112.4

E. coli DnaK (aa 1 - 384)

DAG1708 100 ug
EUR 1098

E. coli DnaJ (aa 1 - 376)

DAG1711 100 µg
EUR 1112.4

E. coli Fur (aa 1 - 148)

DAG1716 100 ug
EUR 1232.4

E. coli NusA (aa 1 - 495)

DAG1717 100 ug
EUR 1098

E. coli SlyD (aa 1 - 196)

DAG1718 100 µg
EUR 1242

E. coli Zwf (aa 1 - 491)

DAG1720 100 µg
EUR 1112.4

E. coli SecB (aa 1 - 155)

DAG1723 100 µg
EUR 1242

E. coli TrxB (aa 1 - 321)

DAG1724 100 ug
EUR 1098

E. coli MBP (aa 1 - 387)

DAG1733 500 ug
EUR 1098

TGF-?1, human recombinant (E. Coli)

7843-10 each
EUR 418.8

TGF-?1, human recombinant (E. Coli)

7843-1000 each
EUR 4822.8

TGF-?1, human recombinant (E. Coli)

7843-50 each
EUR 966

E. coli antibody (FITC)

60-E13 1 ml
EUR 453.6
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)

60-E13B 1 ml
EUR 561.6
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)

60-E14 1 ml
EUR 489.6
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli O157 antibody

10-1490 100 ug
EUR 163.2
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-1521 100 ug
EUR 170.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-E12A 100 ug
EUR 462
Description: Mouse monoclonal E. coli O157 antibody

E. coli DnaJ Antibody

32022-05111 150 ug
EUR 313.2

E. coli pykF Antibody

32163-05111 150 ug
EUR 313.2

E. coli GOR Antibody

32540-05111 150 ug
EUR 313.2

E. coli CysH Antibody

30281-05111 150 ug
EUR 313.2

E. Coli HCP antibody

20R-EG001 1 ml
EUR 1004.4
Description: Goat polyclonal E. Coli HCP antibody

E. coli NDK Antibody

32662-05111 150 ug
EUR 313.2

E. coli PPA Antibody

32678-05111 150 ug
EUR 313.2

E. coli glk Antibody

32864-05111 150 ug
EUR 313.2

E. Coli ldhA Antibody

32898-05111 150 ug
EUR 313.2

E. coli deoC Antibody

32936-05111 150 ug
EUR 313.2

E. coli Skp Antibody

32971-05111 150 ug
EUR 313.2

E. coli nanA Antibody

33092-05111 150 ug
EUR 313.2

E. coli O157 antibody

10C-CR1295M3 100 ug
EUR 470.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli LexA repressor

01-005 50 ug
EUR 574.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor

01-006 250 ug
EUR 1420.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

TesA, E. coli esterase

0306-5 500 Units
EUR 2046

E. coli GroEL Antibody

20012-05011 150 ug
EUR 260.4

E. coli O157 Antibody

abx021684-1mg 1 mg
EUR 1220.4

E. coli O157 Antibody

abx021685-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021686-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021687-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021688-1mg 1 mg
EUR 1128

E. coli O157 Antibody

abx021689-1mg 1 mg
EUR 1195.2

E. coli Antibody (HRP)

abx022972-1ml 1 ml
EUR 718.8

E. coli Antibody (FITC)

abx022974-1ml 1 ml
EUR 777.6

E. coli Antibody (Biotin)

abx022976-1ml 1 ml
EUR 718.8

E. coli Antibody (HRP)

abx022978-1ml 1 ml
EUR 1053.6

E. coli Antibody (Biotin)

abx022979-1ml 1 ml
EUR 886.8

E. coli O157 Antibody

abx024017-1mg 1 mg
EUR 1212

E. coli O 157

abx291002-100mg 100 mg
EUR 744

E. coli FimC [His]

DAG1666 100 ug
EUR 1232.4

E. coli Eco [His]

DAG1667 50 ug
EUR 1232.4

Enterotoxin STp (E. coli)

H-6248.0500 0.5mg
EUR 835.2
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)

H-6248.1000 1.0mg
EUR 1416
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

E. coli Topoisomerase IV

TG2000EC-3 500 units
EUR 819.6

E. coli Topoisomerase IV

TG2000EC-5 1000 units
EUR 1262.4

E. coli DNA Gyrase

TG2000G-3 500 units
EUR 846

E. coli DNA Gyrase

TG2000G-5 1000 units
EUR 1396.8

E. coli DNA Gyrase

TG2000G-7 2000 units
EUR 2203.2

E. coli DNA Ligase

M1217-100 each
EUR 385.2

E. coli Exonuclease I

M1222-100 each
EUR 301.2

E. coli Exonuclease III

M1223-100 each
EUR 320.4

E. coli Recombinant IDNK

P1050-10 each
EUR 196.8

E. coli Recombinant IDNK

P1050-50 each
EUR 457.2

Recombinant E. coli, Glucokinase

P1054-10 each
EUR 196.8

Recombinant E. coli, Glucokinase

P1054-50 each
EUR 457.2

Benzonase, E. Coli Recombinant

7680-25 each
EUR 398.4

Benzonase, E. Coli Recombinant

7680-5 each
EUR 164.4

E. Coli Recombinant TRXB

6331-100 each
EUR 510

E. Coli Recombinant SlyD

6366-100 each
EUR 548.4

E. coli O157 Antibody

V3307-100UG 100 ug
EUR 499
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

E. coli O157 Antibody

V3307-20UG 20 ug
EUR 219
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

E. coli O157 Antibody

V3307SAF-100UG 100 ug
EUR 499
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

Hypoxia/re-oxygenation up-regulated mRNA expression of PLN. Hypoxia and re-oxygenation situation instantly affected human bioengineered cardiac tissue operate. Additional understanding the molecular mechanisms of practical restoration of cardiac tissue after re-oxygenation would possibly present us the brand new perception on coronary heart failure with recovered ejection fraction and preserved ejection fraction.

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